NovaSeq6000
Overview
This service is for sequencing analysis using Illumina Next-Generation Sequencing (NovaSeq6000). Library preparation is conducted by the user (yourself), and SignAC will only perform sequencing and deliver raw data (bcl files) or fastq data. In principle, you will purchase all lanes of the kit (We offer a lane sequencing plan for NovaSeq S4 300 cycles).
| Analysis content | Clusters | Cycles | Output |
|---|---|---|---|
| SP Reagent Kit v1.5 (100 Cycles) | 650–800 M | 100 | 65–80 Gb |
| SP Reagent Kit v1.5 (200 Cycles) | 650–800 M | 200 | 134–167 Gb |
| SP Reagent Kit v1.5 (300 Cycles) | 650–800 M | 300 | 200–250 Gb |
| SP Reagent Kit v1.5 (500 Cycles) | 650–800 M | 500 | 325–400 Gb |
| S1 Reagent Kit v1.5 (100 Cycles) | 1.3–1.6 B | 100 | 134–167 Gb |
| S1 Reagent Kit v1.5 (200 Cycles) | 1.3–1.6 B | 200 | 266–333 Gb |
| S1 Reagent Kit v1.5 (300 Cycles) | 1.3–1.6 B | 300 | 400–500 Gb |
| S2 Reagent Kit v1.5 (100 Cycles) | 3.3 B–4.1 B | 100 | 333–417 Gb |
| S2 Reagent Kit v1.5 (200 Cycles) | 3.3 B–4.1 B | 200 | 667–833 Gb |
| S2 Reagent Kit v1.5 (300 Cycles) | 3.3 B–4.1 B | 300 | 1,000–1,250 Gb |
| S4 Reagent Kit v1.5 (35 Cycles) | 8–10 B | 35 | 280–350 Gb |
| S4 Reagent Kit v1.5 (200 Cycles) | 8–10 B | 200 | 1,600–2,000 Gb |
| S4 Reagent Kit v1.5 (300 Cycles) | 8–10 B | 300 | 2,400–3,000 Gb |
| S4 Reagent Kit v1.5 (300 Cycles) 1 lane | 2B–2.5B | 300 | 600–750 Gb |
Sample Submission Guidelines
Prepared illumina sequence library
- Pool all of the samples, adjust the concentration to 5 times of the installation concentration, and submit at least the specified volume listed below in a 1.5 ml DNA LoBind tube.
- Please write down your name and the Redmine ticket number on your sample tube.
NovaSeq6000 sample concentration
NovaSeq6000 reference installation concentration
NovaSeq6000 NGS sample concentration Standard workflow.
| Library Type | Installation concentration (pM) | Submitted sample concentration (nM) |
|---|---|---|
| PhiX | 250 | 1.25 |
| Illumina DNA PCR-free library pool | 400–600² | 2–3² |
| TruSeq DNA PCR-free library pool | 175–350 | 0.875–1.75 |
| DNA PCR-amplified library pool | 300–600 | 1.5–3.0 |
| Single Cell | 250–500 | 1.25–2.5 |
NovaSeq6000 NGS sample concentration XP (S4 lane sequencing ).
| Library Type | Final Loading Concentration (pM) | Pooled Loading Concentration (nM) |
|---|---|---|
| PhiX | 100 | 0.5 |
| Illumina DNA PCR-free library pool | 300–400 | 1.5–2.0 |
| TruSeq DNA PCR-free library pool | 115–235 | 0.575–1.175 |
| DNA PCR-amplified library pool | 200–400 | 1.0–2.0 |
| Single Cell | 175–275 | 0.875–1.375 |
- Reprinted from llumina NovaSeq6000 Denature and Dilute Libraries Guide
NovaSeq required sample amount
NovaSeq required sample volumes.
| Flow cell type | Required volume |
|---|---|
| SP/S1 | 250 µl or more |
| S2 | 450 µl or more |
| S4 | 700 µl or more |
| S4 lane sequencing | 70 µl or more |
Data Delivery
fastq files (or raw data)
- For Kyoto University researchers: We will provide you with a link to download your data.
- For outside of Kyoto University researchers: We will prepare an encrypted external hard disk drive or flash memory stick with your data.
- Please submit a sample sheet to demultiplex and create fastq files.
Please refer to the Illumina website for instructions on how to create a sample sheet.
- Illumina Experiment Manager
- The sample sheet (sample) can be downloaded from the link below.
Please pay attention to the direction of index 2.
Estimated delivery date
Please contact us.
Reference
1–2 weeks if kits are in stock, 3–4 weeks if not (FY2022 results, except in the case of lane sequencing).