iSeq100
Overview
This service is for sequencing analysis using Illumina Next-Generation Sequencers (iSeq100). Library preparation will be done by yourself, and SignAC will only perform sequencing and deliver raw data (bcl files) or fastq data. In principle, you will purchase all lanes of the kit.
Overview of Illumina iSeq100 Sequence Analysis Service.
| Analysis content | Clusters | Cycles | Output |
|---|---|---|---|
| i1 Reagent v2 (300 Cycles) | 4M | 300 | 1.2 Gb |
Sample Submission Guidelines
Prepared illumina sequence library
- Pool all samples, adjust the concentration to 1 nM, and submit at least 15 µl in a 1.5 ml DNA LoBind tube.
- Please specify the loading concentration on your Redmine ticket.
We often apply 50 pM as a loading concentration for iSeq100. - Please write down your name and the Redmine ticket number down on your sample tube.
Data Delivery
fastq files (or raw data)
- For Kyoto University researchers: We will provide you with a link to download your data.
- For outside of Kyoto University researchers: We will prepare an encrypted external hard disk drive or flash memory stick with your data.
- Please submit a sample sheet to demultiplex and create fastq files.
Please refer to the Illumina website for instructions on how to create a sample sheet.
- Illumina Experiment Manager
- The sample sheet (sample) can be downloaded from the link below.
Please pay attention to the direction of index 2.
Estimated delivery date
Please contact us.
Reference
1–2 weeks if kits are in stock, 3–4 weeks if not (FY2022 results).