NextSeq550
Overview
This service is used for sequencing analysis with Illumina Next-Generation Sequencing (NextSeq550). Library preparation will be done by yourself, and SignAC will only perform the sequence and deliver raw data (bcl files) or fastq data. In principle, you will purchase all lanes of the kit.
| Analysis content | Clusters | Cycles | Output |
|---|---|---|---|
| Mid Output Kit v2.5 (150 Cycles) | Up to 130M | 150 | 16.25–19.5 Gb |
| Mid Output Kit v2.5 (300 Cycles) | Up to 130M | 300 | 32.5–39 Gb |
| High Output Kit v2.5 (75 Cycles) | Up to 130M | 75 | 25–30 Gb |
| High Output Kit v2.5 (150 Cycles) | Up to 400M | 150 | 50–60 Gb |
| High Output Kit v2.5 (300 Cycles) | Up to 400M | 300 | 100–120 Gb |
Sample Submission Guidelines
Prepared illumina sequence library
- Pool all samples, adjust the concentration to 4 nM, 2 nM, 1 nM, or 0.5 nM, and submit at least the specified volume listed below in a 1.5 ml DNA LoBind tube.
- Please specify the loading concentration on your Redmine ticket.
We often apply 1.5–1.8 pM as a loading concentration for NextSeq550.
NextSeq550 required sample amount
| 4 nM | 15 µl or more |
|---|---|
| 2 nM | 30 µl or more |
| 1 nM | 60 µl or more |
| 0.5 nM | 120 µl or more |
- Write your name and the Redmine ticket number down on your sample tube.
Data Delivery
fastq files (or raw data)
- For Kyoto University researchers: We will provide you with a link to download your data.
- For outside of Kyoto University researchers: We will prepare an encrypted external hard disk drive or flash memory stick with your data.
- Please submit a sample sheet to demultiplex and create fastq files.
Please refer to the Illumina website for instructions on how to create a sample sheet.
- Illumina Experiment Manager
- The sample sheet (sample) can be downloaded from the link below.
Please pay attention to the direction of index 2.
Estimated delivery date
Please contact us.
Reference
1–2 weeks if kits are in stock, 3–4 weeks if not (FY2022 results).