• Price List for shared research equipment
• Price List for analysis service

Price List for shared research equipment

iSAL equipment (open to both inside and outside KU) : Please book equipment at KUMaCo.

Equipment Name	Unit	Unit price of usage fee
		ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
10x Genomics Chromium Controller	per sample	11,200	15,100	15,900	31,700
10x Genomics Chromium X	per sample	12,000	17,100	17,700	28,900
Bio-Rad QX200 Droplet Digital PCR system	per sample	900	920	920	1,300
Takara Bio USA SMARTer ICELL8 cx Single-Cell System	per day	16,900	17,000	33,100	57,300
Sage Science PippinHT	per time	800	800	1,400	2,600
Diagenode Megaruptor 3	per time	600	600	700	2,400
Nanopore PromethION24	per slot/day	3,600	6,700	7,600	24,600

• The above prices indicate fees per usage unit (unit price) including consumption tax. The charge will be determined by multiplying this amount (unit price) by the number of usage units.
• The actual cost of flow cells will be additionally charged if provided by SignAC for PromethION24.

ASHBi only equipment : Please book equipment at Share-e.

Equipment Name	Unit		Unit price of usage fee
			ASHBi  (Kyoto University)
Pacific Biosciences Sequel IIe System	per time		35,600
Illumina NextSeq 550 System	per hour		2,000
Illumina NovaSeq 6000 System	per hour		4,200
Illumina iSeq 100 Sequencing System	per time		8,100
Beckman Coulter Biomek i7 Hybrid(MC+Spn-8)	per hour		3,000
ZEISS LSM980 Airyscan 2	per hour		1,500
BD Biosciences Cell Sorter BD FACS Aria Fusion	per hour		2,600
	pre-training per session		60,720
ASHBi Server	per  10TB/1month	Up to 20TB	7,100
		Over 20TB  up to 40TB	9,200
		Over 40TB	11,300

• The above prices indicate fees per usage unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of usage units.

Price List for analysis service

Illumina NextSeq 550 System

Analysis content		Unit	Price
			ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
DNA Sequence Analysis	Mid Output Kit v2.5 (150 Cycles) (Number of clusters: 130 million)	per time	221,400	241,000	245,500	318,600
	Mid Output Kit v2.5 (300 Cycles) (Number of clusters: 130 million)		353,000	385,100	392,400	519,200
	High Output Kit v2.5 (75 Cycles) (Number of clusters: 400 million)		280,400	295,400	298,900	352,500
	High Output Kit v2.5 (150 Cycles) (Number of clusters: 400 million)		520,400	543,300	548,600	636,400
	High Output Kit v2.5 (300 Cycles) (Number of clusters: 400 million)		827,500	865,400	873,500	1,014,900

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• The number of clusters shown in the table is a rough estimate of the number of clusters obtained when using each kit, and is not guaranteed by our analysis.

Illumina NovaSeq 6000 System

Analysis content		Unit	Price
			ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
RNA-seq library preparation and NGS analysis		per１sample	25,300	29,000	30,300	41,400
DNA Sequence Analysis	SP Reagent Kit v1.5 (100 Cycles) (Number of clusters: 800 million)	per time	403,100	449,900	461,500	669,600
	SP Reagent Kit v1.5 (200 Cycles) (Number of clusters: 800 million)		549,600	619,300	636,000	940,100
	SP Reagent Kit v1.5 (300 Cycles) (Number of clusters: 800 million)		693,400	785,500	807,200	1,207,400
	SP Reagent Kit v1.5 (500 Cycles) (Number of clusters: 800 million)		995,100	1,131,500	1,164,200	1,772,500
	S1 Reagent Kit v1.5 (100 Cycles) (Number of clusters: 1.6 billion)		678,700	727,900	739,600	947,700
	S1 Reagent Kit v1.5 (200 Cycles) (Number of clusters: 1.6 billion)		920,800	992,500	1,009,100	1,313,300
	S1 Reagent Kit v1.5 (300 Cycles) (Number of clusters: 1.6 billion)		1,107,800	1,199,800	1,221,600	1,621,800
	S2 Reagent Kit v1.5 (100 Cycles) (Number of clusters: 4.1 billion)		1,505,100	1,566,600	1,580,700	1,836,800
	S2 Reagent Kit v1.5 (200 Cycles) (Number of clusters: 4.1 billion)		1,887,800	1,979,900	2,001,700	2,401,800
	S2 Reagent Kit v1.5 (300 Cycles) (Number of clusters: 4.1 billion)		2,052,100	2,184,100	2,215,100	2,791,400
	S4 Reagent Kit v1.5 (35 Cycles) (Number of clusters: 10 billion)		2,138,000	2,192,600	2,205,100	2,429,200
	S4 Reagent Kit v1.5 (200 Cycles) (Number of clusters: 10 billion)		2,708,100	2,840,000	2,871,000	3,447,300
	S4 Reagent Kit v1.5 (300 Cycles) (Number of clusters: 10 billion)		3,032,400	3,191,600	3,229,300	3,933,600
	S4 Reagent Kit v1.5 (300 Cycles) 1lane (Number of clusters: 2.5 billion)		794,400	838,800	848,800	1,024,800

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• The number of clusters shown in the table is a rough estimate of the number of clusters obtained when using each kit, and is not guaranteed by our analysis.

Illumina iSeq100 System

Analysis content		Unit	Price
			ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
DNA Sequence Analysis	i1 Reagent v2  (300 Cycles） (Number of clusters: 4 million）	per time	113,200	118,300	119,600	132,500

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• The number of clusters shown in the table is a rough estimate of the number of clusters obtained when using each kit, and is not guaranteed by our analysis.

Oxford Nanopore Technologies PromethION24

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
DNA Sequence Analysis	per flow cell	263,100	281,800	285,200	336,300

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Pacific Biosciences Sequel IIe System

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
Whole genome sequence (whole genome library preparation and sequence analysis)	per flow cell	359,800	362,300	405,500	544,000
Iso-Seq library preparation and sequence analysis		355,200	357,700	399,600	535,200
Sequence analysis only		305,600	308,100	350,000	485,500

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Agilent Technologies Femto Pulse System

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
Quality check of long DNA molecules	per sample	4,000	4,000	4,600	6,100

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Agilent 4200 TapeStation

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	Researchers  outside of ASHBi  (Kyoto University)	Academic researchers	Industrial researchers
RNA quality check	per sample	1,200	1,200	1,200	1,300
Quality check of ultra-low amount RNA		1,300	1,300	1,300	1,400
Quality check of NGS libraries together with quantification using Thermo Fisher Qubit System		1,000	1,100	1,100	1,200

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.