Next-generation sequencing analysis services
This service is for sequencing analysis using Illumina Next-Generation Sequencing sequencers (NovaSeq6000, NextSeq550, and iSeq 100). Library preparation will be done by yourself, and SignAC will only perform sequencing and deliver raw data (bcl files) or fastq data. In principle, you will purchase all lanes of the kit (We offer a lane sequencing plan for NovaSeq S4 300 cycles).
| Equipment Name | Analysis content | Clusters | Cycles | Output |
|---|---|---|---|---|
| Illumina NovaSeq 6000 System | SP Reagent Kit v1.5 (100 Cycles) | 650–800 M | 100 | 65–80 Gb |
| SP Reagent Kit v1.5 (200 Cycles) | 650–800 M | 200 | 134–167 Gb | |
| SP Reagent Kit v1.5 (300 Cycles) | 650–800 M | 300 | 200–250 Gb | |
| SP Reagent Kit v1.5 (500 Cycles) | 650–800 M | 500 | 325–400 Gb | |
| S1 Reagent Kit v1.5 (100 Cycles) | 1.3–1.6 B | 100 | 134–167 Gb | |
| S1 Reagent Kit v1.5 (200 Cycles) | 1.3–1.6 B | 200 | 266–333 Gb | |
| S1 Reagent Kit v1.5 (300 Cycles) | 1.3–1.6 B | 300 | 400–500 Gb | |
| S2 Reagent Kit v1.5 (100 Cycles) | 3.3 B–4.1 B | 100 | 333–417 Gb | |
| S2 Reagent Kit v1.5 (200 Cycles) | 3.3 B–4.1 B | 200 | 667–833 Gb | |
| S2 Reagent Kit v1.5 (300 Cycles) | 3.3 B–4.1 B | 300 | 1000–1250 Gb | |
| S4 Reagent Kit v1.5 (35 Cycles) | 8–10 B | 35 | 280–350 Gb | |
| S4 Reagent Kit v1.5 (200 Cycles) | 8–10 B | 200 | 1600–2000 Gb | |
| S4 Reagent Kit v1.5 (300 Cycles) | 8–10 B | 300 | 2400–3000 Gb | |
| S4 Reagent Kit v1.5 (300 Cycles) 1lane | 2–2.5B | 300 | 600–750 Gb | |
| Illumina NextSeq 550 system | Mid Output Kit v2.5 (150 Cycles) | Up to 130M | 150 | 16.25–19.5 Gb |
| Mid Output Kit v2.5 (300 Cycles) | Up to 130M | 300 | 32.5–39 Gb | |
| High Output Kit v2.5 (75 Cycles) | Up to 130M | 75 | 25–30 Gb | |
| High Output Kit v2.5 (150 Cycles) | Up to 400M | 150 | 50–60 Gb | |
| High Output Kit v2.5 (300 Cycles) | Up to 400M | 300 | 100–120 Gb | |
| Illumina iSeq100 System | i1 Reagent v2 (300 Cycles) | 4M | 300 | 1.2 Gb |