RNA QC
Overview
This service analyzes the RNA concentration and RIN analysis on Agilent Technologies’ TapeStation system using either the RNA kit (RNA quality check) or the RNA High Sensitivity kit (ultra-trace RNA quality check).
Sample Submission Guidelines
RNA quality check (TapeStation RNA)
- Sample concentration: 25 ng/µl or more.
- Volume (additional to the RNA-seq analysis, if applicable):4 µl or more.
- Samples should be in 1.5 ml tubes simply and clearly labeled with [# Ticket number], [Name], and [Sample no.].
- We recommend the use of DNA Low bind tubes.
- 8-strip tubes can be used for 8 or more samples, or 96-well plates for 24 or more samples.
Quality check of ultra-low amount of RNA (TapeStation High Sensitivity RNA)
- Sample concentration:0.5 ng/µl or more.
- Volume (Apart from RNA-seq analysis):4 µl or more.
- Samples should be in 1.5 ml tubes simply and clearly labeled with [# Ticket number], [Name], and [Sample no.].
- We recommend the use of DNA Low bind tubes.
- 8-strip tubes can be used for 8 or more samples, or 96-well plates for 24 or more samples.
Data Delivery
Report file (pdf) exported by Agilent software, including RNA concentration and RIN.
- If you require raw data (in a form that can be analyzed by Agilent software), e.g., for smear analysis, please contact us in advance.
Estimated delivery date
Please contact us.
Reference
around 1 week. (FY2022 results).