Equipment

Single cell library preparation system Chromium Controller

10X Genomics
Genome
Cell
Processing
iSAL
SignAC
Med. Bldg. B

Overview

Single-cell NGS library preparation system.
It provides not only single-cell gene expression analysis applications, but also single-cell multiome analysis applications combined with epigenomic analysis, cell surface epitopes profiling, and CRISPR screening.

How to use

Available through KUMaCo.
Fees are charged (Price List).

SignAC provides the following consumables.

ItemItem number
Chromium Dual Index Kit TT set A (96 sample) (10x Genomics)1000215
Chromium Dual Index Kit NT set A (96 sample) (10x Genomics)1000242
Chromium Dual Index Kit NN set A (96 sample) (10x Genomics)1000243
Chromium Dual Index Kit TN set A (96 sample) (10x Genomics)1000250
Pipet-Lite Multi Pipette L8-200XLS+ (METTLER TOLEDO)17013805
Pipet-Lite LTS Pipette L-20XLS+ (METTLER TOLEDO)17014392
Pipet-Lite LTS Pipette L-200XLS+ (METTLER TOLEDO)17014391
Pipet-Lite LTS Pipette L-1000XLS+ (METTLER TOLEDO)17014382
Pipette tip RT LTS 200 µl FL 960A/10 (METTLER TOLEDO)30389240
Pipette tip RT LTS 1000 µl FL 768A/8 (METTLER TOLEDO)30389213
Pipette tip RT LTS 20 µl FL 960A/10 (METTLER TOLEDO)30389226
Pipette tip RT LTS 200 µl FLW FL 960A/10 (METTLER TOLEDO)30389241
Pipette tip RT LTS 1000 µl FLW 768A/8 (METTLER TOLEDO)30389218
Low TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA) (Thermo Fisher Scientific)12090-015
10% Tween 20 (Bio-Rad)1610781
Glycerin (glycerol), 50% (v/v) Aqueous Solution (RICCA Chemical Company)3290-32
Buffer EB (QIAGEN)19086
SPRIselect Reagent Kit 5mL (Beckman Coulter)B23317
PCR Tubes 0.2 ml 8-tube strips (Eppendorf)951010022