Overview
Single-cell NGS library preparation system. High-throughput assay with simultaneous 16-well processing at up to 20,000 cells per well (estimated doublet rate of 8%) without cell multiplex. Also available for low-throughput and standard-throughput assays provided by 10X.
How to use
Available through KUMaCo.
Fees are charged (Price List).
SignAC provides the following consumables.
| Item | Item number |
|---|---|
| Chromium Dual Index Kit TT set A (96 sample) (10x Genomics) | 1000215 |
| Chromium Dual Index Kit NT set A (96 sample) (10x Genomics) | 1000242 |
| Chromium Dual Index Kit NN set A (96 sample) (10x Genomics) | 1000243 |
| Chromium Dual Index Kit TN set A (96 sample) (10x Genomics) | 1000250 |
| Pipet-Lite Multi Pipette L8-200XLS+ (METTLER TOLEDO) | 17013805 |
| Pipet-Lite LTS Pipette L-20XLS+ (METTLER TOLEDO) | 17014392 |
| Pipet-Lite LTS Pipette L-200XLS+ (METTLER TOLEDO) | 17014391 |
| Pipet-Lite LTS Pipette L-1000XLS+ (METTLER TOLEDO) | 17014382 |
| Pipette tip RT LTS 200 µl FL 960A/10 (METTLER TOLEDO) | 30389240 |
| Pipette tip RT LTS 1000 µl FL 768A/8 (METTLER TOLEDO) | 30389213 |
| Pipette tip RT LTS 20 µl FL 960A/10 (METTLER TOLEDO) | 30389226 |
| Pipette tip RT LTS 200 µl FLW FL 960A/10 (METTLER TOLEDO) | 30389241 |
| Pipette tip RT LTS 1000 µl FLW 768A/8 (METTLER TOLEDO) | 30389218 |
| Low TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA) (Thermo Fisher Scientific) | 12090-015 |
| 10% Tween 20 (Bio-Rad) | 1610781 |
| Glycerin (glycerol), 50% (v/v) Aqueous Solution (RICCA Chemical Company) | 3290-32 |
| Buffer EB (QIAGEN) | 19086 |
| SPRIselect Reagent Kit 5mL (Beckman Coulter) | B23317 |
| PCR Tubes 0.2 ml 8-tube strips (Eppendorf) | 951010022 |