Masataka Nakaya

Research Overview

Development of a next generation of primate gene modification technology

The reproductive and developmental engineering in cynomolgus monkeys is less technically advanced than that in mice due to the difficulty of reproductive management and lack of resources. Our objective is to develop a new genetic modification technology using mice and apply them quickly to cynomolgus monkeys in order to produce primate model animals.

Biography

Masataka Nakaya obtained his PhD from Kindai University (2017). He was appointed Assistant Professor in 2017 in Shiga University of Medical Science.

Publications

Jayakumar V., Nishimura O., Kadota M., Hirose N., Sano H., Murakawa Y., Yamamoto Y., Nakaya M., Tsukiyama T., Seita Y., Nakamura S., Kawai J., Sasaki E., Ema M., Kuraku S., Kawaji H., Sakakibara Y., Chromosomal-scale de novo genome assemblies of Cynomolgus Macaque and Common Marmoset. Scientific data, 8(1), 159, 2021.

Kojima Y., Yamashiro C., Murase Y., Yabuta Y., Okamoto I., Iwatani C., Tsuchiya H., Nakaya M., Tsukiyama T., Nakamura T., Yamamoto T., Saitou M., GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program. Life science alliance, 4(5), 2021.

Seita Y., Morimura T., Watanabe N., Iwatani C., Tsuchiya H., Nakamura S., Suzuki T., Yanagisawa D., Tsukiyama T., Nakaya M., Okamura E., Muto M., Ema M., Nishimura M., Tooyama I., Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein. Journal of Alzheimer's disease, JAD, 75(1), 45-60, 2020.

Tsukiyama T.#, Kobayashi K.#, Nakaya M.#, Iwatani C., Seita Y., Tsuchiya H., Matsushita J., Kitajima K., Kawamoto I., Nakagawa T., Fukuda K., Iwakiri T., Izumi H., Itagaki I., Kume S., Maegawa H., NishinakamuraI R., Nishino S., Nakamura S, Kawauchi A, Ema M., Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease. Nature communications, 10(1), 5517, 2019. (# Equal contribution)

Seita Y., Tsukiyama T., Azami T., Kobayashi K., Iwatani C., Tsuchiya H., Nakaya M., Tanabe H., Hitoshi S., Miyoshi H., Nakamura S., Kawauchi A., Ema M., Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys. Biology of Reproduction, 100(6), 1440-1452, 2019.

Nakaya M., Tanabe H., Takamatsu S., Hosokawa M., Mitani T., Visualization of the spatial arrangement of nuclear organization using three-dimensional fluorescence in situ hybridization in early mouse embryos: A new "EASI-FISH chamber glass" for mammalian embryos. The Journal of reproduction and development , 63(2), 167-174, 2017.

Awards

JRD Outstanding Paper Award (2017)