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Sample pre-processing
Pippin HT (size selection of DNA fragments)

Overview

With this service, SignAC performs size selection of DNA fragments provided by users using the PippinHT system from Sage Science. This service is suitable for both short (≤1.5 kb) and long (≥6 kb) DNA fragments.
We perform quality control (QC) before and after size selection.

  • The service name specified in our regulations：
    DNA断片抽出（1.5kb以下）
    DNA断片抽出（6kb以上）

Sample submission guidelines

DNA sample
  • Volume: minimum 50 µl ~ (sufficient for at least two reactions)
  • Total amount: 40 ng to 40 µg. Each 20 µl reaction requires 15 ng to 15 µg DNA.
  • Concentration: minimum 750 pg/µl (~75 ng/µl is recommended)
Samples should be in 1.5 ml tubes being simply and clearly labeled with [# Ticket number], [Name], and [Sample no.].   
We recommend the use of DNA low-bind tubes.

  • When making an inquiry, please specify the aimed size range of the DNA fragments to be recovered and attach a list of sample names and gel electrophoresis images.
  • PippinHT enables size selection within the following ranges: A to E (sizes are approximate). Please feel free to consult us.   
Example of sample list (Excel)

※Please note that pre- and post-extraction DNA QC is included in the fee for each sample.
※If the DNA concentration measured by Qubit falls below the lower limit of the specified range, sufficient recovery may not be guaranteed, but the fee will still apply.

Data delivery

Extracted samples
Analysis results (pdf) of samples before and after extraction.

Estimated delivery date

Please contact us.

Reference

1 week

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