• Price List for shared research equipment
• Price List for analysis service

Price List for shared research equipment

iSAL equipment (open to both inside and outside KU) : Please book equipment at KUMaCo.

Equipment Name	Unit	Unit price of usage fee
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA  (Kyoto University)	Academic researchers	Industrial researchers
10x Genomics Chromium Controller	per sample	11,200	15,100	15,100	15,900	31,700
10x Genomics Chromium X	per sample	12,200	17,400	17,400	18,000	29,100
Bio-Rad QX200 Droplet Digital PCR system	per sample	900	920	920	920	1,300
Takara Bio USA SMARTer ICELL8 cx Single-Cell System	per day	16,900	17,000	17,000	33,100	57,300
Sage Science PippinHT	per time	1,700	1,700	1,700	2,800	5,300
Diagenode Megaruptor 3	per time	800	800	800	900	2,500
SPT Labtech   Dragonfly Discovery (Standard Volume)	per time	3,200	4,400	4,400	4,600	7,800
SPT Labtech  Dragonfly Discovery (Low Volume)	per time	3,700	4,800	4,800	5,000	8,200
Nanopore PromethION24	per slot / day	3,700	6,800	6,800	7,700	24,700

• The above prices indicate fees per usage unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of usage units.
• The actual cost of flow cells will be additionally charged if provided by SignAC for PromethION24.

ASHBi only equipment : Please book equipment at Share-e.

Equipment Name	Unit		Unit price of usage fee
			ASHBi  (Kyoto University)
Pacific Biosciences Sequel IIe System	per time		35,600
Illumina NextSeq 550 System	per hour		2,000
Illumina NovaSeq 6000 System	per hour		4,200
Illumina iSeq 100 Sequencing System	per time		8,100
Beckman Coulter Biomek i7 Hybrid(MC+Spn-8)	per hour		3,000
ZEISS LSM980 Airyscan 2	per hour		1,500
BD Biosciences Cell Sorter BD FACS Aria Fusion	per hour		2,600
	pre-training per session		60,720
ASHBi Server	per  10TB/1month	Up to 60TB	5,500
		Over 60TB up to 120TB	7,600
		Over 120TB	9,800

• The above prices indicate fees per usage unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of usage units.

ASHBi・CiRA only equipment : Please book equipment at Share-e.

Equipment Name	Unit		Unit price of usage fee
			ASHBi  (Kyoto University)	CiRA  (Kyoto University)
Pacific Biosciences Revio System	per cell		84,000	84,000

• The above prices indicate fees per usage unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of usage units.

Price List for analysis service

Illumina NextSeq 550 System

Analysis content		Unit	Price
			ASHBi  (Kyoto University)	CiRA  (Kyoto University)	Researchers outside of ASHBi・CiRA  (Kyoto University)	Academic researchers	Industrial researchers
DNA Sequence Analysis	Mid Output Kit v2.5 (150 Cycles) (Number of clusters: 130 million)	per time	230,400	250,700	250,700	255,100	324,800
	Mid Output Kit v2.5 (300 Cycles) (Number of clusters: 130 million)		366,400	398,600	398,600	405,700	526,400
	High Output Kit v2.5 (75 Cycles) (Number of clusters: 400 million)		291,500	307,500	307,500	310,900	361,900
	High Output Kit v2.5 (150 Cycles) (Number of clusters: 400 million)		539,700	563,300	563,300	568,400	652,000
	High Output Kit v2.5 (300 Cycles) (Number of clusters: 400 million)		857,400	897,100	897,100	904,900	1,039,500

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• The number of clusters shown in the table is a rough estimate of the number of clusters obtained when using each kit, and is not guaranteed by our analysis.

Illumina NovaSeq 6000 System

Analysis content		Unit	Price
			ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
RNA-seq library preparation and NGS analysis		per１sample	26,200	30,700	30,700	32,000	43,100
Genome DNA Methylation Analysis using New England Biolab EM-seq Kit and Illumina NovaSeq		per sample	230,400	239,600	239,600	250,900	293,700
DNA Sequence Analysis	SP Reagent Kit v1.5 (100 Cycles) (Number of clusters: 800 million)	per time	416,800	463,800	463,800	475,100	676,500
	SP Reagent Kit v1.5 (200 Cycles) (Number of clusters: 800 million)		567,600	638,500	638,500	654,700	949,100
	SP Reagent Kit v1.5 (300 Cycles) (Number of clusters: 800 million)		715,500	806,200	806,200	827,300	1,214,600
	SP Reagent Kit v1.5 (500 Cycles) (Number of clusters: 800 million)		1,026,000	1,159,600	1,159,600	1,191,300	1,780,000
	S1 Reagent Kit v1.5 (100 Cycles) (Number of clusters: 1.6 billion)		702,000	753,200	753,200	764,500	965,900
	S1 Reagent Kit v1.5 (200 Cycles) (Number of clusters: 1.6 billion)		951,700	1,022,700	1,022,700	1,038,900	1,333,200
	S1 Reagent Kit v1.5 (300 Cycles) (Number of clusters: 1.6 billion)		1,144,400	1,235,200	1,235,200	1,256,300	1,643,500
	S2 Reagent Kit v1.5 (100 Cycles) (Number of clusters: 4.1 billion)		1,557,000	1,618,100	1,618,100	1,631,800	1,879,700
	S2 Reagent Kit v1.5 (200 Cycles) (Number of clusters: 4.1 billion)		1,951,800	2,042,600	2,042,600	2,063,700	2,450,900
	S2 Reagent Kit v1.5 (300 Cycles) (Number of clusters: 4.1 billion)		2,120,300	2,249,900	2,249,900	2,280,000	2,837,700
	S4 Reagent Kit v1.5 (35 Cycles) (Number of clusters: 10 billion)		2,212,300	2,266,800	2,266,800	2,278,900	2,495,800
	S4 Reagent Kit v1.5 (200 Cycles) (Number of clusters: 10 billion)		2,799,100	2,928,700	2,928,700	2,958,800	3,516,500
	S4 Reagent Kit v1.5 (300 Cycles) (Number of clusters: 10 billion)		3,133,700	3,289,600	3,289,600	3,326,200	4,007,800
	S4 Reagent Kit v1.5 (300 Cycles) 1lane (Number of clusters: 2.5 billion)		822,100	866,700	866,700	876,400	1,046,800

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• The number of clusters shown in the table is a rough estimate of the number of clusters obtained when using each kit, and is not guaranteed by our analysis.

Illumina iSeq100 System

Analysis content		Unit	Price
			ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
DNA Sequence Analysis	i1 Reagent v2  (300 Cycles） (Number of clusters: 4 million）	per time	117,700	123,400	123,400	124,700	136,800

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• The number of clusters shown in the table is a rough estimate of the number of clusters obtained when using each kit, and is not guaranteed by our analysis.

Oxford Nanopore Technologies PromethION24

Analysis content	Unit	Price
		ASHBi (Kyoto University）	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Ultra-Long DNA sequence analysis	per flow cell	300,600	309,900	309,900	329,000	380,100
Sequence analysis of fragmented DNA using SQK-LSK114 and PromethION24	per flow cell・sample	239,100	248,400	248,400	267,000	319,500
Library preparation from fragmented DNA using SQK-LSK114	per sample	41,200	41,200	41,200	41,800	43,300
DNA sequence analysis using the Rapid Sequencing kit and PromethION 24	per flow cell・sample	221,100	230,400	230,400	248,200	299,200
DNA library preparation using the Rapid Sequencing kit	per sample	23,200	23,200	23,200	23,200	23,200
Direct RNA Sequence Analysis	per flow cell	245,800	255,100	255,100	273,100	324,100
DNA Sequence Analysis (Sequence run only of pre-existing DNA libraries)	per flow cell	200,000	209,300	209,300	227,200	278,300
Flow cell wash and library reloading	per time	15,100	15,100	15,100	15,100	15,100

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Pacific Biosciences Revio System

Analysis content	Unit	Price
		ASHBi (Kyoto University）	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Whole genome DNA sequence (whole genome DNA library preparation and sequence analysis)	per sample・cell	382,600	382,600	464,400	509,000	783,800
Whole genome DNA library preparation only	per sample	62,200	62,200	62,200	64,100	70,200
RNA library preparation and sequence analysis	per sample・cell	390,100	390,100	471,900	515,200	785,500
RNA library preparation only	per sample	69,700	69,700	69,700	70,300	71,900
Kinnex full-length RNA library preparation and sequence analysis	per sample・cell	502,700	502,700	584,500	629,000	902,300
Kinnex full-length library preparation only	per sample	180,700	180,700	180,700	182,500	187,100
Kinnex scRNA library preparation and sequence analysis	per sample・cell	480,900	480,900	562,600	607,200	880,400
Kinnex scRNA library preparation only	per sample	158,900	158,900	158,900	160,700	165,200
DNA sequence analysis (Sequence run only of pre-existing DNA libraries)	per cell	324,200	324,200	406,000	448,700	717,500

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Pacific Biosciences Sequel IIe System

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Whole genome sequence (whole genome library preparation and sequence analysis)	per flow cell	359,800	362,300	362,300	405,500	544,000
Iso-Seq library preparation and sequence analysis		355,200	357,700	357,700	399,600	535,200
Sequence analysis only		305,600	308,100	308,100	350,000	485,500

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Agilent Technologies Femto Pulse System

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Quality check of long DNA molecules	per sample	4,000	4,000	4,000	4,600	6,100

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Agilent 4200 TapeStation

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
RNA quality check	per sample	1,200	1,200	1,200	1,200	1,300
Quality check of ultra-low amount RNA		1,300	1,300	1,300	1,300	1,400
Quality check of NGS libraries together with quantification using Thermo Fisher Qubit System		1,000	1,100	1,100	1,100	1,200

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

10x Genomics Chromium X High-Throughput System

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Single-Cell Gene Expression Analysis Using the Fixed RNA Kit	per time	657,900	686,600	686,600	692,800	804,800

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Sage Science PippinHT

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
DNA fragment extraction (under 1.5 kb）	per time	23,800	24,000	24,000	25,100	27,800
DNA fragment extraction (over 6 kb)	per time	30,400	30,400	30,400	32,700	38,200

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Diagenode Megaruptor3

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Shearing long DNA	per time	8,800	8,800	8,800	8,900	10,500

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.

Genome DNA Extraction from Cells

Analysis content	Unit	Price
		ASHBi  (Kyoto University)	CiRA (Kyoto University)	Researchers outside of ASHBi・CiRA (Kyoto University)	Academic researchers	Industrial researchers
Genome DNA Extraction from Cells (Alcohol Precipitation Method)	per sample	9,600	9,600	9,600	10,200	11,700
High Molecular Weight Genome DNA Extraction from Cells (Glass Beads Method)	per sample	10,900	10,900	10,900	11,500	13,000

• The above prices indicate fees per service unit (unit price) including consumption tax.
• The charge will be determined by multiplying this amount (unit price) by the number of service units.
• ‘The genome DNA extraction from cells (alcohol precipitation method)’ and ‘high molecular weight genome DNA extraction from cells (glass beads method)’ are sample preparation services solely for the following analyses. Therefore, they can only be accepted when requested simultaneously with any of the corresponding analyses:
  • The analyses using the Oxford Nanopore Technologies PromethION24
  • The analyses using the Pacific Biosciences Revio System
  • Genome DNA Methylation Analysis with New England Biolab EM-seq Kit and Illumina NovaSeq