PacBio whole-genome sequencing
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides whole-genome sequencing using the Sequel IIe or Revio system. Users submit genomic DNA or metagenomic samples, and SignAC prepares libraries using the PacBio SMRTbell Prep Kit 3.0 from the samples and generates HiFi reads using the Sequel IIe or Revio system.
- The service name specified in our regulations：
全ゲノム配列解析（全ゲノムライブラリーの調製とシーケンス解析）
全ゲノム配列解析（全ゲノムライブラリの調製のみ）
Sample submission guidelines
Genomic DNA
- Volume: minimum 8 µl
- Total amount: minimum 7 µg
- Concentration: minimum 60 ng/µl
※Please quantify your sample using a Qubit fluorometer.
※We recommend high-molecular-weight DNA, that is, >50 kb (70% ≧ 10 kb; GQN10kb ≧ 7.0 and 50% ≧ 30 kb; GQN30kb ≧ 5.0)
※We recommend the quality check using Femto Pulse. SignAC offers QC service (link).
※The presence of contaminants may adversely affect library preparation.
※If the genomic DNA sample has a high level of DNA damage, sufficient data may not be obtained.
※Please submit your samples in 1.5 mL tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- As a general rule, proceed with library preparation based on the concentration specified by each individual.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
hifi_reads.bam files
- Output: ~30 Gb (Sequel IIe)/~90 Gb (Revio)
We do not guarantee the data amount. - Please note that this service provides analysis on a per-SMRT cell basis. We offer “library preparation only” as an additional option, allowing for multiplex analysis of multiple libraries within a single SMRT cell.
- Please contact us if you need 5mC detection data.
Estimated delivery date
Please contact us.
Reference
2–3 weeks if kits are in stock; 3–4 weeks if not.