PacBio sequencing only
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service is for sequencing runs only of pre-prepared SMRTbell libraries on the Sequel IIe or Revio system.
- The service name specified in our regulations:
PacBio シーケンス解析のみ
Sample submission guidelines
Pre-prepared (pooled) SMRTbell-library: no less than 350 ng/pool (Sequel IIe)/no less than 700 ng/pool (Revio)
※Please quantify your sample using a Qubit fluorometer.
※Please submit your samples in 1.5 mL tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- As a general rule, we proceed with library preparation based on the concentration specified by the users.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
hifi_reads.bam files
- Output: ~2 million reads (Sequel IIe)/~6 million reads (Revio)
We do not guarantee the data amount. - Please contact us if you need 5mC detection data.
Estimated delivery date
Please contact us.
Reference
2–3 weeks if kits are in stock; 3–4 weeks if not.