PacBio transcriptome analysis (Kinnex single-cell RNA)
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides full-length cDNA sequencing using the PacBio Kinnex single-cell RNA kit and MAS-Seq ligation. Users submit single-cell cDNA samples before fragmentation prepared using the 10× Chromium Single Cell 3′- or 5′ kit. We will construct 16-plex full-length cDNA libraries and perform sequencing on the Revio system. The delivered data will be HiFi reads segmented at the ligation adapter sequences.
- The service name specified in our regulations：
Kinnex-scRNAライブラリーの調製とシーケンス解析
Kinnex-scRNAライブラリーの調製のみ
Sample submission guidelines
10x cDNA (3′ or 5′)
- Minimum: 50 ng
- Please consult us if you have a limited amount of samples.
- Recommended average segment length: 500–1100 bp
※Please submit your samples in 1.5 mL tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- As a general rule, proceed with library preparation based on the concentration specified by each individual.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
- Segmented.bam
- Output: ~30 million/90 million reads after segmentation. We do not guarantee the data amount.
- Please note that this service provides analysis on a per-SMRT cell basis. We offer “library preparation only” as an additional option, allowing for multiplex analysis of multiple libraries within a single SMRT cell.
- Please feel free to consult us for basic primary analysis (such as mapping). We may be able to accommodate your request.
Estimated delivery date
Please contact us.
Reference
2–3 weeks if kits are in stock; 3–4 weeks if not.