PacBio transcriptome analysis (Kinnex full-length RNA)
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides full-length RNA sequence analysis with higher throughput using the Sequel IIe or Revio system. Users submit total RNA samples, and SignAC prepares an 8-plex full-length cDNA library using the PacBio Kinnex full-length RNA kit with the MAS-Seq ligation method. Sequencing is then performed on the Revio system. The delivered data will be HiFi reads segmented at the ligation adapter sequence.
- The service name specified in our regulations:
Kinnex-full length RNAライブラリーの調製とシーケンス解析
Kinnex-full lengthライブラリーの調製のみ
Sample submission guidelines
Total RNA
- Volume: minimum 8 µl
- Total amount: minimum 700 ng
- Concentration: minimum 60 ng/µl
※Please quantify your sample using a Qubit fluorometer.
※≥7.0 RIN
※Because reverse transcription is performed using oligo(dT) primers, RNA from organisms lacking poly(A) tails cannot be analyzed.
※Please submit your samples in 1.5 mL tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- As a general rule, we proceed with library preparation based on the concentration specified by the user.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
hifi_reads.bam files or segmented.bam
- Output: ~45 million reads (Revio) after segmentation
We do not guarantee the data amount. - Please note that this service provides analysis on a per-SMRT cell basis. We offer “library preparation only” as an additional option, allowing for multiplex analysis of multiple libraries within a single SMRT cell.
- Please consult us for a simple primary analysis (mapping, etc.).
Estimated delivery date
Please contact us.
Reference
2–3 weeks if kits are in stock; 3–4 weeks if not.