PacBio transcriptome analysis (Iso-seq)
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides full-length RNA sequence analysis using the Sequel IIe or Revio system. Users submit total RNA samples, and SignAC prepares libraries using the PacBio Iso-seq library and generates HiFi reads.
- The service name specified in our regulations:
RNAライブラリーの調製とシーケンス解析
RNAライブラリの調製のみ
Sample submission guidelines
Total RNA
- Volume: minimum 8 µl
- Total amount: minimum 700 ng
- Concentration: minimum 60 ng/µl
- Please quantify your sample using a Qubit fluorometer.
- ≥7.0 RIN
- Because reverse transcription is performed using oligo(dT) primers, RNA from organisms lacking poly(A) tails cannot be analyzed.
- Please submit your samples in 1.5 ml tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- As a general rule, we proceed with library preparation based on the concentration specified by the users.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
hifi_reads.bam files
- Output: ~2 million reads (Sequel IIe)/~6 million reads (Revio)
We do not guarantee the data amount. - Please note that this service provides analysis on a per-SMRT cell basis. We offer “library preparation only” as an additional option, allowing for multiplex analysis of multiple libraries within a single SMRT cell.
- Please contact us for a simple primary analysis (mapping, etc.).
Estimated delivery date
Please contact us.
Reference
2–3 weeks if kits are in stock; 3–4 weeks if not.