ONT ultra-long-read DNA sequencing
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
We offer a service that prepares ultra-long-read libraries (using the Oxford Nanopore Technologies SQK-ULK114 kit) from your frozen cell pellets, sequences them on R10.4.1 flow cells, and delivers the sequencing data. We may perform up to two washes and reloads of the flow cell for a maximum of three loads. This service enables the analysis of ultra-high-molecular-weight DNA sequences (N50: ~80 kb).
- The service name specified in our regulations：
ウルトラロングリードDNA解析
Sample submission guidelines
- Two tubes containing pellets of >6 million cells each.
- Please dissociate the cells using an appropriate method and count the cell number before submission.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
POD5/FASTQ files
- Output: 25–100 Gb/single flow cell.
We do not guarantee the data amount. - The sequencing portion is a per-flow cell analysis service.
- FASTQ files will be generated by the latest version of MinKnow’s default Basecaller with high-accuracy mode.
- Please contact us if you need 5mC detection data.
- Please consult us for a simple primary analysis (such as mapping).
Estimated delivery date
Please contact us.
Reference
2–3 weeks if kits are in stock; 3–4 weeks if not.