Long-read anlaysis Epigenome analysis

ONT DNA sequencing (ligation kit)

Overview
Sample Submission Guidelines
Price list
Data Delivery
Estimated delivery date
Related services

Overview

This service provides an ONT DNA sequencing (ligation kit) analysis using PromethION24 (Oxford Nanopore Technologies). Users submit DNA samples (genomic DNA, amplicon, etc.). SignAC prepares libraries using the Oxford Nanopore Technologies Ligation Sequencing Kit V14 (SQK-LSK114), loads it onto an R10.4.1 flow cell once, and returns the resulting sequencing data to the users. If you need flow cell washing and library reloading during the sequencing run, please add the “ONT flow cell washing and library reloading” service (availability may depend on the amount of the prepared library).

The service name specified in our regulations：
SQK-LSK114とPromethION24による断片化DNAのシーケンス解析
SQK-LSK114による断片化DNAのライブラリ調製

Sample submission guidelines

≥2 µg (or 200–300 fmol) DNA

Please contact us if you can only provide samples of smaller amounts.
We recommend gDNA with OD_260/280 > 1.8 and OD_260/230 > 2.0–2.2 as measured by Nanodrop.

※Please submit your samples in 1.5 mL tubes. We recommend using DNA low-bind tubes.

Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.

Example of sample list (Excel)

Add a serial number to the sample name and write the number to the tube as well.
As a general rule, we proceed with library preparation based on the concentration specified by users.
Please write your name and the Redmine ticket number on your sample tube.

Data delivery

POD5/fastq files

Output: 25–100 Gb/single flow cell.
We do not guarantee the data amount.
The sequencing portion is a per-flow cell analysis service. You can also add the “library preparation only” option to multiplex multiple libraries within a single flow cell.
fastq files will be generated by the latest version of MinKnow’s default Basecaller with high-accuracy mode.
Please contact us if you need 5mC detection data.
Please contact us for a simple primary analysis (such as mapping).