ONT direct RNA sequencing
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides direct RNA sequencing using the Oxford Nanopore Technologies direct RNA sequencing kit (SQK-RNA004). Customers provide your total RNA or poly(A)+ RNA sample, and we prepare a library, load it onto an RNA flow cell (FLO-PRO004RA) once, perform sequencing, and deliver the sequencing data.
This method allows the analysis of RNA sequences without the amplification bias introduced by PCR. Additionally, RNA base modifications can also be analyzed.
Please note that wash and reload are not available for this service.
- The service name specified in our regulations：
Direct RNAシーケンス解析
Sample submission guidelines
750 ng poly(A)+ RNA or 2.5 μg total RNA in 20 μl.
- Please contact us if you can only provide samples of smaller amounts.
- Poly(A)+. RNA submission is recommended. As we primarily use oligo(dT) primers for reverse transcription, RNA from organisms without poly(A) tails cannot be analyzed.
※Please submit your samples in 1.5 ml tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- As a general rule, proceed with library preparation based on the concentration specified by each individual.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
POD5/fastq files
- Output: 5 –20 million reads/single flow cell.
We do not guarantee the data amount. - The sequencing portion is a per-flow cell analysis service.
- FASTQ files will be generated by the latest version of MinKnow’s default Basecaller with high-accuracy mode.
- Detection of some base modifications may be possible.
Estimated delivery date
Please contact us.
Reference
1–3 weeks if kits are in stock; 3–4 weeks if not.