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Sample pre-processing
Genome DNA extraction

Overview

This service provides genomic DNA extraction from your frozen cell pellets and a subsequent quality check using Femto Pulse.

Depending on your experimental needs, we offer two extraction methods: alcohol precipitation and glass bead precipitation. The glass bead method is generally recommended for obtaining longer DNA fragments.

  • Genomic DNA extraction (alcohol precipitation):
    The Promega Wizard® Genomic DNA Purification Kit (#A1120) is used for standard genomic DNA extraction.
  • High-molecular-weight genomic DNA extraction (glass bead precipitation):
    For longer DNA fragments, we recommend the NEB Monarch® HMW DNA Extraction Kit for Cells & Blood (#T3050).

Example of extracted DNA size distribution

  • The service name specified in our regulations：
    細胞試料からのゲノム抽出（アルコール沈澱法）
    細胞試料からの高分子ゲノム抽出（ガラスビーズ法）

Sample submission guidelines

Cell pellet

※Please submit your cell pellet after dissociation using a suitable method for your cell type, followed by washing with PBS and freezing in liquid nitrogen.
※Please ship the samples on dry ice.
※Please ensure each 1.5 ml tube contains at least 100,000 cells.
※We recommend submitting at least two tubes of cell pellets if possible.
※Please note that this service is only available for users who intend to use the extracted genomic DNA for subsequent SignAC EM-seq, PacBio whole-genome sequencing, or ONT DNA sequencing.

  • Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
Example of sample list (Excel)
  • Add a serial number to the sample name and write the number to the tube as well.
  • Please write your name and the Redmine ticket number on your sample tube.

Data delivery

Genomic DNA (may not be retained for future use in other services)
DNA QC report (pdf)

※Please collect the extracted genomic DNA from SignAC or arrange for a prepaid shipping service.

Estimated delivery date

Please contact us.

Reference

4 weeks

Related services

ONT DNA sequencing (ligation kit)
ONT DNA sequencing (rapid kit)
PacBio whole-genome sequencing
Pippin HT (size selection of DNA fragments)
Megaruptor 3 (DNA shearing)