Whole-genome methylome (EM-seq)
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides deep sequence data for DNA methylation analysis in single-base resolution. Users submit genomic DNA, which SignAC will convert to libraries using the New England Biolab NEBNext® Enzymatic Methyl-seq Kit (E7120) to be sequenced with NovaSeq6000. FASTQ data will be delivered as the product.
- The service name specified in our regulations:
New England Biolab社EM-seqキットとイルミナ社NovaSeqによるゲノムDNAメチル化解析
Sample submission guidelines
Genomic DNA
- Volume: minimum 8 µl
- Total amount: minimum 150 ng, recommended ~450 ng
- Concentration: minimum 5 ng/µl
- Please quantify your sample using a Qubit fluorometer.
- The presence of contaminants may adversely affect library preparation.
- Fragmentation conditions may vary depending on the genomic DNA length. Consider our long-read DNA quality-control service.
- Please submit your samples in 1.5 ml tubes. We recommend using DNA low-bind tubes.
- Please submit an Excel file with the sample name (information written on the tube) and concentration through the Redmine system.
- Add a serial number to the sample name and write the number on the tube cap and/or wall as well.
- As a general rule, proceed with library preparation based on the concentration specified by each individual.
- Please write your name and the Redmine ticket number on your sample tube.
Data delivery
FASTQ files
Sequencing is targeted at >250 million reads per sample, aiming at 300 million reads, with 150 bp paired-end reads.
Adapter trimming is not performed.
Estimated delivery date
Please contact us.
Reference
2 months