Overview
A system for droplet digital PCR. It performs PCR reaction in 20,000 partitioned droplets and digitally counts numbers of droplets that have amplification of targets, achieving absolute quantification of target DNA fragments. It can be applied to a wide range of genetic studies including gene expression analysis, detection of rare mutations, and so on.
Features
Quantification of low concentrations is also possible even if difficult with conventional qPCR.
Making droplets in maximum 8 wells at one time.
Reactions and measurements of droplets can be done in 96 wells.
How to use
Available through KUMaCo.
Usage fee will be charged (Price List).
SignAC explains how to use it.
SignAC provides the following consumables.
| Consumables SignAC provides | Cat.No. |
|---|---|
| DG8 Cartridges (/8well) (BioRad) | 186-4008 |
| DG8 Gaskets (/8well) (BioRad) | 186-3009 |
| DG8 Cartidges and Gasket set (BioRad) | 186-4007 |
| ddPCR Supermix for probes (no dUTP) (BioRad) | 186-3023 |
| Droplet generation oil for probes (BioRad) | 186-3005 |
| ddPCR Droplet Reader Oil (BioRad) | 1863004 |
| Plates (case of 25) (Eppendorf) | 0030.128.591 |
| Plates (dividable) (Eppendorf) | 0030.133.404 |
| Buffer control (BioRad) | 186-3052 |
| Pierceable Foil Heat Seal (BioRad) | 1814040 |
| 200 µl pipet tips (Rainin) | 30389239 |