Single cell library preparation system Chromium Controller (10X Genomics)
Overview
Single-cell NGS library preparation system.
It provides not only single-cell gene expression analysis applications, but also single-cell multiome analysis applications combined with epigenomic analysis, cell surface epitopes profiling, and CRISPR screening.
How to use
Available through KUMaCo.
Fees are charged (Price List).
SignAC provides the following consumables.
| Item | Item number |
| Chromium Dual Index Kit TT set A (96 sample) (10x Genomics) | 1000215 |
| Chromium Dual Index Kit NT set A (96 sample) (10x Genomics) | 1000242 |
| Chromium Dual Index Kit NN set A (96 sample) (10x Genomics) | 1000243 |
| Chromium Dual Index Kit TN set A (96 sample) (10x Genomics) | 1000250 |
| Pipet-Lite Multi Pipette L8-200XLS+ (METTLER TOLEDO) | 17013805 |
| Pipet-Lite LTS Pipette L-20XLS+ (METTLER TOLEDO) | 17014392 |
| Pipet-Lite LTS Pipette L-200XLS+ (METTLER TOLEDO) | 17014391 |
| Pipet-Lite LTS Pipette L-1000XLS+ (METTLER TOLEDO) | 17014382 |
| Pipette tip RT LTS 200µL FL 960A/10 (METTLER TOLEDO) | 30389240 |
| Pipette tip RT LTS 1000µL FL 768A/8 (METTLER TOLEDO) | 30389213 |
| Pipette tip RT LTS 20µL FL 960A/10 (METTLER TOLEDO) | 30389226 |
| Pipette tip RT LTS 200µL FLW FL 960A/10 (METTLER TOLEDO) | 30389241 |
| Pipette tip RT LTS 1000µL FLW 768A/8 (METTLER TOLEDO) | 30389218 |
| Low TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA) (Thermo Fisher Scientific) | 12090-015 |
| 10% Tween 20 (Bio-Rad) | 1610781 |
| Glycerin (glycerol), 50% (v/v) Aqueous Solution (RICCA Chemical Company) | 3290-32 |
| Buffer EB (QIAGEN) | 19086 |
| SPRIselect Reagent Kit 5mL (Beckman Coulter) | B23317 |
| PCR Tubes 0.2 ml 8-tube strips (Eppendorf) | 951010022 |