Oxford Nanopore Technologies (ONT) PromethION24 overview
We offer a service for analyzing DNA or RNA sequences using the Oxford Nanopore Technologies (ONT) PromethION24 sequencer. Depending on the sample type and users’ demands, we provide the following five applications:
(1) ONT ultra-long-read DNA sequencing
- The service name specified in our regulations：
ウルトラロングリードDNA解析
(2) ONT DNA sequencing (ligation kit)
- The service name specified in our regulations：
SQK-LSK114とPromethION24による断片化DNAのシーケンス解析
SQK-LSK114による断片化DNAのライブラリ調製
(3) ONT DNA sequencing (rapid kit)
- The service name specified in our regulations：
Rapid Sequencing kitとPromethION 24によるDNAシーケンス解析
Rapid Sequencing kitによるDNAライブラリ調製
(4) ONT direct RNA sequencing
- The service name specified in our regulations：
Direct RNAシーケンス解析
(5) ONT sequencing only
- The service name specified in our regulations：
DNAシーケンス解析（調整済みDNAライブラリーのシーケンスランのみ）
(6) ONT flow cell wash and library reloading
- The service name specified in our regulations：
フローセル洗浄とライブラリー再ロード
For application (2) ONT DNA sequencing (ligation kit) and application (3) ONT DNA sequencing (rapid kit), we offer both library preparation only and comprehensive services that include library preparation and sequencing. For application (5) ONT sequencing only, we provide sequencing services for pre-prepared libraries.
By flexibly combining these services, it is possible to prepare multiple libraries for analysis in a single flow cell or analyze a single library in multiple flow cells.
Libraries are basically sequenced continuously for 72 hours from the start. By adding “(6) ONT flow cell washing and library reloading,” we can recover pore activity and increase data yield during the 72-hour run. For application (1) ONT ultra-long-read DNA sequencing, “ONT flow cell wash and library reloading” is performed within the analysis service by default.