Single-cell fixed RNA gene expression analysis (10× Flex)
- Overview
- Sample Submission Guidelines
- Price list
- Data Delivery
- Estimated delivery date
- Related services
Overview
This service provides single-cell fixed RNA gene expression analysis. Users submit fixed cells or nuclei samples. SignAC processes the cells or nuclei, prepares single-cell RNA-seq libraries using 10X Genomics Chromium X and Flex kits, conducts sequencing, and returns FASTQ data.
Sample submission guidelines
Cell pellets (prepared with the following method)
- Species: human or mouse
※Please suspend cells in quenching buffer, add 10% enhancer, further add 50% glycerol to a final concentration of 10% glycerol, and store at −80°C. (Quenching buffer and enhancer are included in the Single-Cell Fixed RNA Sample Preparation Kit.)
| Sample Type | Recommended # |
|---|---|
| Fixed cells | 200,000-2 x 106 |
| Fixed nuclei | 400,000-2 x 106 |
| FFPE dissociated suspension | 400,000-2 x 106 |
※Fixation, dissociation, and cryopreservation: Please use the 10x Genomics Chromium Next GEM Single-Cell Fixed RNA Sample Preparation kit (No. 1000414). Refer to the following links for protocols.
- Please submit an Excel file with the sample name (information written on the tube) and cell count through the Redmine system.
- Add a serial number to the sample name and write the number to the tube as well.
- Please write your name and the Redmine ticket number on your sample tube.
※Please submit your samples in 1.5 mL tubes. We recommend using DNA low-bind tubes.
Data delivery
FASTQ files
- Sequencing is targeted at >200 million reads per sample, aiming at 300 million reads.
- Please order an additional sequencing service provided by SignAC to further increase the sequencing depth after the primary data delivery.
- Please consult us for a simple primary analysis (mapping, etc.).
- In principle, libraries prepared by SignAC will not be returned.
Estimated delivery date
Please contact us.
Reference
6 weeks