Automatic cell washing system with no need of centrifugation. Laminar flow generated by double nozzles allows mild cell washing. Up to 8 samples can be processed at one time.

• Reduces cell damage and cell loss
• A small number of cells can be washed (100 cells/well)
• Short processing time (6 minutes for 16 samples)
• High cleaning capacity
• Improved single-cell sequencing data due to reduced background

• Available to ASHBi PI group members. Reservations are required.
• Please provide consumables (16-well strips, tips, and washing buffer) yourself.
• Required solutions: 70% ethanol (1% Tween20), water (1% Tween20), and cell washing buffer. Please prepare them yourself.

Usage rules

• Please leave usage logs.
• When using 70% ethanol (containing 1% Tween 20), water (containing 1% Tween 20), and cell washing buffer, be sure to treat them with a 0.22 µm filter before use. (To prevent clogging of equipment due to debris)
• When you prepare washing buffers in reagent bottles that can be attached to the buffer inlet bottle cap (GL45 standard screw-top bottles), you may reuse the cap.
• If the system has not been used for more than one month, priming with 70% ethanol (1% Tween 20) should be performed twice instead of once at the startup.
• When you apply cells with strong adhesion or cells containing a large amount of debris, please wash them with PURELOX diluted 12 times with MilliQ (SignAC will prepare) at the shutdown.
• Wash reagent bottles (including buffer inlet bottle caps and tubes) with MilliQ after use.
• Dispose of waste liquid after use.