Hiiragi Group

Developmental Biology

Hiiragi Group

Research Overview

A defining feature of living systems is the capacity to break symmetry and generate well-defined forms and patterns through self-organisation. Our group aims to understand the design principle of multi-cellular self-organisation using early mammalian embryos. Our studies revealed that morphogenesis and gene expression in the early development are highly dynamic and stochastically variable in space and time (Figure 1). Determining how embryos robustly establish a size, shape and pattern at the right time despite the preceding variability remains a fundamental open question. We have recently developed an experimental framework that integrates biology, physics and mathematics (Figure 2). We aim to understand how molecular, cellular and physical signals are dynamically coupled across the scales for tissue self-organisation. Furthermore, we will identify developmental mechanisms shared and distinct among mammalian species. To this end, we will first establish the early primate developmental atlas at single-cell resolution by integrating single-cell omics data into 4D morphogenetic and lineage map derived from advanced microscopy. We adopt a wide variety of experimental strategies including embryology, genetics, advanced microscopy, biophysics and theoretical modelling in order to address fundamental questions in development and cell biology at molecular, cellular and systems levels.

Figure 1

Figure 1: Molecular heterogeneity during mouse blastocyst patterning. Cells expressing Nanog (green), Gata6 (red) or Serpinh1 (blue).

Figure 2

Figure 2: Symmetry breaking in the mouse embryo at the 8-cell stage (the emerging apical domain marked with Ezrin, red; Actin, green).


Takashi Hiiragi
Principal Investigator

Takashi Hiiragi

Laboratory Website
  • Mei Makino

    Dispatched Employee

  • Marina Hamaji

    Dispatched Employee


Ichikawa, T., Zhang, H. T., Panavaite, L., Erzberger, A., Fabrèges, D., Snajder, R., Wolny, A., Korotkevich, E., Tsuchida-Straeten, N., Hufnagel, L., Kreshuk, A., & Hiiragi, T. (2022). An ex vivo system to study cellular dynamics underlying mouse peri-implantation development. Developmental Cell, 57(3), 373–386.e9.

Chan, C. J., Costanzo, M., Ruiz-Herrero, T., Mönke, G., Petrie, R. J., Bergert, M., Diz-Muñoz, A., Mahadevan, L., & Hiiragi, T. (2019). Hydraulic control of mammalian embryo size and cell fate. Nature, 571(7763), 112–116.

Niwayama, R., Moghe, P., Liu, Y. J., Fabrèges, D., Buchholz, F., Piel, M., & Hiiragi, T. (2019). A Tug-of-War between Cell Shape and Polarity Controls Division Orientation to Ensure Robust Patterning in the Mouse Blastocyst. Developmental Cell, 51(5), 564–574.e6.

Korotkevich, E., Niwayama, R., Courtois, A., Friese, S., Berger, N., Buchholz, F., & Hiiragi, T. (2017). The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo. Developmental Cell, 40(3), 235–247.e7.

Maître, J. L., Turlier, H., Illukkumbura, R., Eismann, B., Niwayama, R., Nédélec, F., & Hiiragi, T. (2016). Asymmetric division of contractile domains couples cell positioning and fate specification. Nature, 536(7616), 344–348.

Contact T.Hiiragi

    This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.